Fig. 6.

Effects of TRF and purified T3 on PERK, ATF-6, and IRE-1α activity. T3 (10 μg/ml) affect the UPR intracellular pathway in HeLa cells. CC indicates the treatment with the vehicle only (DMSO). Brefeldin (BFA) (2.5 μg/ml) was used as a positive control of EndoR stress. α-Tubulin was used as the loading control. a T3 treatment has no effect on the expression of ATF-6 and PERK proteins at 24 h from the treatment. b IRE-1 protein expression and phosphorylation are significantly affected by T3 treatment. The figure shows one representative experiment out of at least three separate experiments. Densitometry and statistical analysis is showed as Additional file 3: Figure S2