Fig. 9.

XBP-1 activation in a ERβ silenced clone of breast cancer cells (MCF-7). A specific clone of MCF-7 cells, not expressing ERβ, was incubated with δ-T3 (20 μg/ml), γ-T3 (10 μg/ml), α-TOC, and α-T3 (20 μg/ml). Details about Pst1 digestion are provided in the “Methods” section. CC indicates treatment with the vehicle only (DMSO). Brefeldin (BFA) was used as a positive control of EndoR stress induction. The figure shows one representative experiment out of at least three separate experiments