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. Author manuscript; available in PMC: 2016 Dec 23.
Published in final edited form as: RSC Adv. 2012 Jan 23;2(9):3670–3677. doi: 10.1039/C2RA01376B

Fig. 7.

Fig. 7

Biochemical characterization of cBMV–R5. (A) Transmission electron micrograph of cBMV–R5. (B) Size exclusion chromatogram of cBMV–R5 monitored at 260 nm (blue), 280 nm (red) (Abs 260 : 280 nm = 1.7). Intact cBMV–R5 fractions were collected and further characterized. (C) SDS-PAGE of cBMV and cBMV–R5 visualized under white light after Coomassie staining. Modified coat proteins (CP–R5) have an increased molecular weight. The ratio between CP–R5 : CP was determined based on band intensity and found to be 1 : 3. (D) Western blot probed with streptavidin–alkaline phosphatase detecting the biotin-tag of the R5 peptide.