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. 2016 Dec 21;4:e2532. doi: 10.7717/peerj.2532

Figure 3. Expression and purification of BipC.

Figure 3

(A) Purification of fusion protein His-BipC using Ni-NTA affinity chromatography. Lane M1, PageRuler prestained protein ladder; lane 1, uninduced pET30a(+)::bipC; lane 2, induced pET30a(+)::bipC; lane 3, cell lysate of induced pET30a(+)::bipC; lane 4, storage buffer; lane5, protein flow-through; lane 6–7, wash samples; lane 8–12, elution of purified His-BipC protein on 12% SDS-PAGE. (B) Size exclusion chromatography purification of His-BipC protein. Lane M2, Precision Plus protein ladder; lane 1, purified BipC protein.