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(A) Percentage of Lactate dehydrogenase (LDH) released was assayed in order to determine the cytotoxicty in A549 human lung epithelial cells exposed to BipC. The percentage of LDH released is directly proportional to the concentration of BipC. (B) A549 epithelial cells exposed to (ii) 50 µg/ml, (iii) 500 µg/ml, (iv) 1,000 µg/ml of purified BipC, and (i) untreated cells (control) viewed under the light microscope.
