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. 2016 Dec 21;4:e2532. doi: 10.7717/peerj.2532

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©2016 Kang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.

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Samples containing 2 mM monomeric actin (10% Pyrene-actin) and fluorescence (expressed in Arbitrary Units, AU) were measured over time after initiation of polymerisation by His-BipC. (A) Actin polymerization of His-BipC at different concentrations and (B) actin depolymerization. Velocities were determined for the interval of 600–1200 s using the results of two independent experiments.