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. 2016 Dec 21;4:e2532. doi: 10.7717/peerj.2532

Figure 7. In vitro interaction analysis between His-BipC, F-actin, and G-actin by pull-down assays.

Figure 7

This protein pull-down assay demonstrated a possible interaction between BipC and both of the monomeric and filamentous actin. Lane M1, PageRuler prestained protein ladder; lane 1, His-BipC protein was immobilized on His-beads, followed by incubation with F-actin; lane 2, purified His-BipC; lane 3, purified His-BipC incubated with empty PBS buffer, lane 4, F-actin; lane 5, G-actin; lane 6, His-BipC protein with G-actin; lane 7, actin was immobilized on His-beads (control).