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. Author manuscript; available in PMC: 2016 Dec 23.
Published in final edited form as: Science. 2015 Feb 12;347(6226):1148–1152. doi: 10.1126/science.aaa4136

Fig. 1. Overall architecture of the CNC.

Fig. 1

(A) Domain structures of the yeast coat nups and sAB-57. Black lines indicate the crystallized fragments. U: unstructured, D: domain invasion motif, VH: heavy chain variable region, CH: heavy chain constant region, VL: light chain variable region, CL: light chain constant region. (B) Reconstitution of the yeast CNC•sAB-57, lacking Nup133. Elution profiles from a Superdex 200 10/300 column are shown for Nup120•Seh1•Nup85 (Trimer 1), Sec13•Nup145C•Nup84NTD (Trimer 2), CNC, and CNC•sAB-57 (left). SDS-PAGE gel of the reconstituted CNC•sAB-57 used for crystallization (right). (C) Cartoon and schematic representations of the yeast CNC•sAB-57 crystal structure viewed from two sides.