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. Author manuscript; available in PMC: 2017 Sep 30.
Published in final edited form as: Circ Res. 2016 Aug 1;119(8):921–930. doi: 10.1161/CIRCRESAHA.116.309281

Figure 4. Expression of SDF1α and CXCR4 during derivation of CSCs from iPSCkit.

Figure 4

A, Schematic of the experimental design. B, Confocal immunofluorescence illustrates that the generation of CSCs from iPSCkit involves migration of CSCs from the embryoid bodies (EBs, solid line). C-D, high magnification of the area delineated by the inset in panel B, illustrates co-localization of EGFP with Nkx2.5 in the migrated CSC derivatives. E-F Gene-expression analysis during differentiation of iPSCkit to CSCs shows a dramatic upregulation in SDF1α and CXCR4. n=3; Scale bars, 100μm.