Figure 1. Rickettsiella contigs in initial assembly.

Initial assembly of all Trachelipus rathkei sequencing data seems to contain contigs coming from two distinct Rickettsiella lineages. (A) One set of contigs displays high similarity to R. grylli NZ_AAQJ00000000 (red dots), while the contigs in the other set are more divergent (blue dots), but contigs in both sets seem to span the entire length of the R. grylli genome. (B) The two sets of contigs also form two distinct clusters based on sequencing depth in the two T. rathkei samples; the high similarity contigs are present at moderate depth in the male sample and very low depth in the female sample, while the low similarity contigs are present at very high depth in the female sample and low depth in the male sample; only a small number of contigs seem to be mis-classified.