| Name | Sequence | Note |
|---|---|---|
| SubpoolAmp_Fwd | GGCCAGTGAGCTCGACAAGTTTCAG | |
| SubpoolAmp_Rev | GTGACGGGCAAATCACTAACCACCC | |
| GuidePoolAmp_Fwd | GGCTTTATATATCTTGTGGAAAGGACGAAACACCG | |
| GuidePoolAmp_Rev | CTTATTTAAACTTGCTATGCTGTTTCCAGCATAGCTCTTAAAC | |
| IlluminaPoolSeq_Fwd | AATGATACGGCGACCACCGAGATCTACA NNNN CGATTTCTTGGCTTTATATATCTTGTGG |
Ns denote sequencing barcode |
| IlluminaPoolSeq_Rev | CAAGCAGAAGACGGCATACGAGAT NNNNNNNN ACAGTCGAGGCTGATCAGC |
Ns denote sequencing barcode |
| CustomRead1 Primer | CAAGCAGAAGACGGCATACGAGAT NNNNNNNN ACAGTCGAGGCTGATCAGC |
|
| CustomRead3 Primer | CGATTTCTTGGCTTTATATATCTTGTGGAAAGGACGAAACACCG | |
| CustomIndex Primer | GCTGATCAGCGGGTTTAAACGGGCCCTCTAGG | |
| CassetteAmp_Fwd | CCCGTTTAAACCCGCTGATCAGCCTCGACTGT | |
| CassetteAmp_Rev | TCGCCAGGGTTTTCCCAGTCACGACGCTTAATTAAGCTTGTGCCCCAGT |
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