| 1 rxn | |
|---|---|
| Q5 2X master mix | 25 |
| P7 primer @10uM | 1.25 |
| pBA439_rev @ 10uM | 1.25 |
| Template (5ng total) | x |
| water (qs up to final rxn of 50ul) | y |
| Total | 50 |
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