Fig. 7.

The bri1-301 mutant showed reduced sensitivity to ABA for stomatal closure. Different concentrations of ABA (1 μM, 100 nM, and 10 nM) were added to the opening solution and the seedlings were incubated for the indicated times. Stomatal closure was measured as the width/length ratio of the cotyledon stomata. Experiments were independently repeated three times (n=25 each time). Error bars indicate standard errors (*P<0.0001, compared with the Col-0 plants under the same treatment, t-test). (This figure is available in color at JXB online.)