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Uptake of radiolabeled amino acids by 22Δ10α yeast cells. Empty: cells transformed with the empty vector pDR196-Ws from which the gateway cassette has been removed. AAP3: cells expressing AAP3. AAP3 + UMAMIT14: cells co-expressing AAP3 and UMAMIT14 carried on a single vector. Uptake of 2 mM of Gln (A), Pro (B), Ala (C), Leu (D), Lys (E), or Asp (F) was examined. Error bars show standard deviation (n=3 technical replicates).
