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. 2016 Nov 17;67(22):6385–6397. doi: 10.1093/jxb/erw412

Fig. 5.

Fig. 5.

Phloem transfer assay. Distribution of the radioactivity absorbed as [3H]Gln (A) and [14C] sucrose (B) in shoots, roots, and the growth medium of plants after 4 h of feeding. One mature leaf of 5-week-old plants grown in hydroponics was soaked in J medium containing labeled compounds. The fed leaf was removed from the shoots prior to counting. WT, wild-type. Significant differences (P<0.05) are indicated by different letters according to one-way ANOVA in conjunction with Tukey’s test (n=3 biological replicates).