Fig. 5.
Phloem transfer assay. Distribution of the radioactivity absorbed as [3H]Gln (A) and [14C] sucrose (B) in shoots, roots, and the growth medium of plants after 4 h of feeding. One mature leaf of 5-week-old plants grown in hydroponics was soaked in J medium containing labeled compounds. The fed leaf was removed from the shoots prior to counting. WT, wild-type. Significant differences (P<0.05) are indicated by different letters according to one-way ANOVA in conjunction with Tukey’s test (n=3 biological replicates).