Fig. 6.

Glutamine and other amino acids secreted by the wild-type (WT), umamit14 and umamit18 mutants. Plants were grown for 2 weeks in liquid J medium supplemented with 20 mM KNO₃ and 30 mM sucrose. The medium was then replaced with fresh J medium and collected after 3 d for analysis. Significant differences (P<0.05) are indicated by different letters according to one-way ANOVA in conjunction with Tukey’s test (n=6 biological replicates). Contents for individual amino acids from the same dataset are presented in Supplementary Table S5. Amino acid contents were normalized against the dry weight of seedlings in each sample.