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. 2016 Nov 9;67(22):6445–6458. doi: 10.1093/jxb/erw415

Fig. 6.

Fig. 6.

Whole-mount RNA in situ hybridizations of YUC6 (A–D) in Col-0 seedlings, and histochemical GUS analysis in the ASA1::GUS transgenic line (Col-0 background) (E–L) at 22 DAS of growth in the presence/absence of IBA and/or ACC. (A) Hybridization signal of the antisense probe in a domed ARP under HF. (B, C) Weak hybridization signal in a domed ARP under ACC alone (B), and under IBA alone (C, arrow). (D) Almost absent hybridization signal in an ARP under IBA+ACC. Insets in (A) and (B) show the absence of hybridization signal in domed ARPs of the samples treated with the sense probe. (E, F) ASA1 expression at the base of an early domed ARP (E) and strong expression in the apex of a mature AR (F) (HF treatment). (G, H) Weak ASA1 expression under ACC alone at the base of an early domed ARP (G), and at the base and, faintly, in the apex of a mature AR (H). (I, J) ASA1 expression at the base of a domed ARP (I) and at the tip of a mature AR (J) (IBA alone). (K, L) Highly reduced gene expression at the base of a domed ARP (K), and at the tip of a mature AR (L) under IBA+ACC. Scale bars are 40 μm.