Table 1.
Mean values (±SE) of IAA and IBA levels (ng g−1 FW) in hypocotyls with ARs excised from Col-0, ein3eil1, and tir1afb2 seedlings at 22 DAS of growth under darkness either in the absence of exogenous hormones (HF), or in the presence of 0.1 μM ACC (ACC), or 10 μM IBA (IBA), or 0.1 μM ACC plus 10 μM IBA (IBA+ACC). Different lower-case letters indicate significant differences within the same genotype and auxin type, whereas no letter or an asterisk indicates no significant difference. Different upper-case letters indicate significant differences between genotypes within the same treatment and auxin type, whereas no letter indicates the absence of significant differences. n=3 replicates per genotype and treatment.
| Treatments | Col-0 | ein3eil1 | tir1afb2 |
|---|---|---|---|
| IAA (ng g−1 FW) | |||
| HF | 131.36 ± 3.83 | 191.31 ± 3.83 A | 141.50 ± 4.10 |
| ACC | 201.80 ± 8.44 a | 197.99 ± 6.07 | 196.10 ± 4.11 a |
| IBA | 227.30 ± 4.55 b | 234.84 ± 4.70 f,* | 144.06 ± 6.03 A |
| IBA+ACC | 310.03 ± 6.20 c,A | 212.14 ± 4.25 * | 210.70 ± 12.26 a,c |
| IBA (ng g−1 FW) | |||
| HF | 10.18 ± 0.73 | 10.12 ± 0.51 | 8.08 ± 0.85 |
| ACC | 23.74 ± 1.13 a,A | 9.44 ± 0.47 | 11.37 ± 0.97 |
| IBA | 33.28 ± 1.66 d | 28.09 ± 1.90 d,B,* | 12.07 ± 0.43 A |
| IBA+ACC | 28.58 ± 0.59 e | 29.00 ± 2.19* | 9.33 ± 1.17 A |
a P<0.0001 difference with respect to HF treatment; b P<0.0001 difference with respect to HF, and P<0.05 difference with respect to ACC alone; c P<0.0001 difference with respect to IBA alone; d P<0.0001 difference with respect to HF and ACC alone; e P<0.0001 difference with respect to HF, and P<0.05 difference with respect to IBA alone and ACC alone; f P<0.001 difference with respect to HF, and P<0.01 difference with respect to ACC alone; A P<0.0001 difference with respect to the other genotypes; B P<0.05 difference with respect to Col-0.