Skip to main content
. 2016 Apr 7;25(12):2393–2403. doi: 10.1093/hmg/ddw106

Figure 3.

Figure 3.

Mutations in the 5′-UTR influence translational efficiency of the SERPINF1-012 transcript. (A) Fragments containing the wild-type or mutant 5′-UTRs of the SERPINF1-012 transcript were cloned into the pGL4.10 expression vector and transfected into MG-63 cells. (B) For the luciferase assay wild-type values were set to 1, to which mutant values were normalized. These results are the average of 12 repeats in four independent experiments. The c.440-40_440-38delTCG (3bpDel) and c.441G > C mutations show a significant reduction in translational activity, whereas the c.601G > A mutation displays a significant increase. Error bars indicate standard error of mean (*P < 0.05; **P < 0.01 in a Student’s two-tailed t-test versus control data).