Figure 2.

Respiratory activity of brain non-synaptic mitochondria isolated from 6- to 8-week-old WT (thin traces) and R6/2 (thick traces) mice. In A and B are representative traces of mitochondrial O₂ consumption for mitochondria at 37 °C in incubation medium supplemented with pyruvate (3 mM) plus malate (1 mM) or succinate (3 mM) plus glutamate (3 mM), respectively. Arrows indicate the addition of either non-synaptic mitochondria (Mtc), 200 µM ADP, or 60 µM 2,4-DNP. In (C) and (D) are statistical analyses of respiratory rates. Data are presented as mean ± SEM from 5 separate experiments. Here and in all other experiments with isolated mitochondria, three WT and three transgenic R6/2 mice were used in each experiment.