Figure 3. Pten Deletion Promotes Satellite Cell Proliferation and Differentiation during Perinatal Muscle Growth.

(A) Immunofluorescence of Pax7 and Ki67 in cross-sections of hind limb muscles from newborn (P1) WT and Pten^MKO mice.

(B–C) Quantification of Pax7⁺ cell (B) and Pax7⁺Ki67⁺ cell (proliferating, C) number in cross-sections of hind limb muscles (n=3).

(D) Immunofluorescence of MyoG and dystrophin in cross-sections of hind limb muscles.

(E) Quantification of MyoG⁺ cell number in cross-sections of hind limb muscles (n=3).

(F) Western blot analysis of myogenic and proliferating markers in skeletal muscles from P7 mice. Arrow indicates the right band.

Scale bar, 50 µm. Data are shown as mean ± SEM (t-test: *P<0.05; **P<0.01).

Also see Figure S2.