Fig.2.

Pharmacodynamic response with LBH589 treatment. A-B) Western blots showing levels of histone H3 acetylation in cortical tissue (A) and muscle (B) of R6/2 mice treated with LBH589 or vehicle. Mice were treated with LBH589 at the dose rate of 20 mg/kg/three times per week for 3 weeks and acetylation levels were determined two hrs after the last injection. LBH589 treatment significantly increases total H3 acetylation in both brain and muscle tissue. GAPDH and Actin are used as loading controls and the level of AcH3 was normalized to total histone H3 level. n = 4-5. P value ^*p < 0.05; ^**p < 0.01. Error bars = S.E.