Fig.4.
Sensitivity of HBLAK cells towards cytotoxic treatment. (a) Molecular response to cellular stress induced by UV radiation (40 J/m2) or the indicated concentrations of Cisplatin, Etoposide and Doxorubicin (all 24 h) by western blot analysis of p53 and p21. α-Tubulin was detected as a loading control. The UC cell line 5637 and cells transfected with a p21 plasmid served as positive controls for p53 and p21, respectively. (b) Sensitivity of HBLAK towards treatment with Cisplatin for 72 h determined by MTT assay. Mean results from triplicate assays are shown. IC50 concentrations ranged around 5 μM. For comparison TERT-NHUC cells and three different passages of a primary NHUC cultured were treated accordingly.