Table 1. Data collection and refinement statistics for PCNA:TIP and PCNA:FEN-1.
| PCNA withTIP (PDB ID: 5DA7) | PCNA with FEN-1 peptide (PDB ID: 5DAI) | |
|---|---|---|
| Diffraction data | ||
| space group | F222 | R3:H |
| cell dimensions (a,b,c) (Å) | 82.34, 184.08, 330.68 | 152.07, 152.07, 35.24 |
| resolution range (Å) | 30.0–2.8 | 28.8–2.0 |
| no. measured intensities | 514,688 | 66,808 |
| no. unique reflections | 30,707 (2,720)a | 20,143 (2,077) |
| average redundancy | 19.8 (6.3) | 3.31 (3.14) |
| % completeness | 98.9 (89.0) | 98.2 (97.9) |
| Rmerge | 0.116 (0.825) | 0.043 (0.419) |
| average I/sigI | 36.6 (2.2) | 11.9 (2.2) |
| Wilson B-factor | 80.6 | 43.7 |
| Refinement | ||
| resolution limits (Å) | 30.0–2.8 | 28.8–2.0 |
| R-factor (95% of the data) | 0.201 (0.300) | 0.182 (0.274) |
| Rfree (5% of the data) | 0.261 (0.359) | 0.224 (0.331) |
| rmsd bond lengths (Å) | 0.010 | 0.008 |
| rmsd bond angles (º) | 1.36 | 1.14 |
| Ramachandran favored (%) | 93.4 | 97.7 |
| Ramachandran outliers (%) | 1.8 | 0.0 |
| no. polypeptide chains in asymmetric unit | 2 PCNA, 2 TIP proteins | 1 PCNA, 1 FEN-1 peptide |
aNumbers in parentheses denote the values for the highest resolution shell.