Figure 5.

The increased expression of Oct4a, Nanog, and Ceacam6 proteins found in MCF-7 monolayers upon long-term exposure to ethanol is considerably reduced or disappears in mammospheres. Mammospheres were obtained as described and then further incubated for 1 week in the presence or absence of 25 mM ethanol and were analyzed by western blot analysis and corrected for GAPDH expression. Top, expression of Oct4a (45 kDa), Nanog (39 kDa); and Ceacam6 (42 kDa), with GAPDH as reference. Bottom, densitometric determinations; ^*p<0.05.