Figure 4.

MALAT1 regulated miR-506 by direct targeting.

Notes: (A) A wt-MALAT1 3′-UTR luciferase-reporter vector (wt-MALAT1) and mut-MALAT1 3′-UTR luciferase-reporter vectors (mut-MALAT1) were constructed by sequentially mutating the two predicted two miR-506-binding sites in the MALAT1 3′-UTR. (B) The wt-MALAT1/mut-MALAT1 vectors and miR-506 NC/miR-506 mimics were cotransfected into SKOV3 cells. Luciferase activity of the MALAT1 3′-UTR luciferase-reporter vector was significantly reduced in miR-506 mimic-transfected cells compared to control groups. miR-506-mediated repression of MALAT1 3′-UTR luciferase-reporter activity was abolished by mutation of the putative miR-506-binding site in the MALAT1 3′-UTR. Data presented as mean ± standard deviation of three independent experiments. **P<0.01.

Abbreviations: miR, microRNA; wt, wild type; UTR, untranslated region; mut, mutant; NC, negative control.