Figure 5. The beta sheet scaffold and nucleotide-binding site of Gαi1 are deprotected in the nucleotide-free complex with Ric-8A.
(a) kinetics of HDX at selected Gαi1 peptides in Gαi1•GDP, light gray, and Gαi1•Ric-8A, dark gray; error bars represent the standard deviation computed for three technical replicates (see Materials and methods) (b) section of the beta sheet and α5 in the Ras domain that abut the nucleotide-binding site, colored according to the extent of HDX, using the coloring scheme used in Figure 4; (c) elements of the nucleotide-binding site featuring the hydrophobic cluster between αG, β4, β5 and α4. Tube diameter is proportional to deuteration. Side chains and GDP are shown as stick figures: carbon, gray, oxygen, red and nitrogen, blue.