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. 2016 Nov 28;5:e19090. doi: 10.7554/eLife.19090

Figure 6. Transposable element landscape.

(A) Proportion of DNA annotated as TEs (black) or unannotated (white) using RepeatMasker (Smit et al., 2015) and a custom putative TE library (see text). MAC putative TE content is about 1 Mb, potentially corresponding to a mixture of TE sequences retained in the MAC assembly and repeats not corresponding to TEs still in the library. (B) Proportion of putative TEs by class for MIC (ALL and high-confidence IESs) and MAC. In MIC(ALL), the most abundant elements (besides unclassified) correspond to DNA TEs (‘cut-and-paste’, Mavericks and Tlr elements). More than half of the MIC(ALL) non-LTR elements could be annotated as LINE1 elements. (C) Evolutionary view of putative TEs in the MIC. For each class, amounts of DNA are shown as a function of the percentage of divergence to the consensus (by bins of 1%), as a proxy for age: the older the TE invasion, the more copies will have accumulated mutations (higher percentage of divergence, right of the graph). Conversely, sequences corresponding to youngest elements show little divergence (left of the graph).

DOI: http://dx.doi.org/10.7554/eLife.19090.017

Figure 6—source data 1. Tetrahymena putative TE library.
Consensus sequences of putative TEs of the Tetrahymena germline genome (fasta format): combination of de novo repeat library and TEs from the literature. See 'Materials and methods', 'Repeat Analysis'.
DOI: 10.7554/eLife.19090.018
Figure 6—source data 2. Details of putative TEs contribution to the MIC chromosome super-assemblies.
Detailed metrics for each putative TE contribution to Tetrahymena MIC chromosomes super-assemblies, such as numbers of fragments, total amount, average divergence to consensus (proxy for age), etc. Obtained by parsing the RepeatMasker output (.out file), using https://github.com/4ureliek/Parsing-RepeatMasker-Outputs/blob/master/parseRM_simple.pl.
DOI: 10.7554/eLife.19090.019
Figure 6—source data 3. Putative TE annotation of high-confidence 7551 IESs.
Raw RepeatMasker output of the high confidence set of 7551 IESs, with two additional columns: the IES identifier (as in Supplementary file 3A) and the total putative TE content of each IES.
DOI: 10.7554/eLife.19090.020

Figure 6.

Figure 6—figure supplement 1. MAC retention of TE termini.

Figure 6—figure supplement 1.

Figure 6—figure supplement 2. Landscape details of DNA TEs.

Figure 6—figure supplement 2.