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. Author manuscript; available in PMC: 2016 Dec 23.
Published in final edited form as: Methods. 2015 Jan 5;76:149–161. doi: 10.1016/j.ymeth.2014.12.018

Figure 12.

Figure 12

Joint NMR/ITC analysis of AAC(6′)-Ii binding to AcCoa (A-C) and paromomycin (D-E). Fraction of enzyme in the 0-bound, 1 bound and 2 bound states determined by ITC (A,D). Intensities of a representative apo peak (dashed line) and holo peak (solid line) throughout a titration (B, E). Histograms of the relative contributions of the 1 bound state to apo (I1apo) and holo (I1holo) peaks of AAC(6′)-Ii (C,F).