Figure 6.

Schematic representation of trapezoidal integration of ΔH_A^app/RT². The points represent the fitted values of ΔH_A^app, divided by the corresponding values of RT². The difference in binding affinity between temperatures T_i and T_ii corresponds to the shaded area, i.e. K_A^app(ii) = K_A^app(i) + shaded area. Thus the ΔH_A^app/RT² profile together with the single value of K_A^app(i) specifies the affinity constants at all other temperatures. Adapted from Freiburger et al [30].