Skip to main content
. Author manuscript; available in PMC: 2018 Jan 1.
Published in final edited form as: Mol Microbiol. 2016 Oct 27;103(1):67–85. doi: 10.1111/mmi.13544

Fig. 1. The flagellin proteins of T. denticola are glycosylated.

Fig. 1

(A) SDS-PAGE (left panel) and immunoblotting (right panel) analyses of isolated PFs from the wild-type strain. 2-D electrophoresis (B) and immunoblotting (C) analyses of the isolated PFs from the wild-type strain. Purified PFs from the wild-type strain were analyzed using 2D-gel electrophoresis, followed by glycosylation staining (GS) (D) and lectin blots with ConA (E) and LFA (F). For the immunoblotting, the blots were probed with the T. pallidum FlaB antiserum (αFlaB) and T. denticola FlaA antiserum (αFlaA). For the lectin blots, the blots were probed with either ConA (Concanavalin A) or LFA (Limax Favus Agglutinin). A (FlaA); B1 (FlaB1); B2 (FlaB2); and B3 (FlaB3).