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. Author manuscript; available in PMC: 2018 Jan 1.
Published in final edited form as: Mol Microbiol. 2016 Oct 27;103(1):67–85. doi: 10.1111/mmi.13544

Fig. 4. High-resolution mass spectrometry analysis of the 450.2 Da glycan.

Fig. 4

nLC-HCD MS/MS analysis of the T108-123 (see sequence in Table 1) FlaB3 glycopeptide ion at m/z 1177.6: (A) full m/z range and (B) zoomed-in area showing the glycan oxonium ions plus neighboring peptide fragment ions. The best fitting elemental formulae for the m/z 451.1922 and m/z 275.1231 were C18O11H31N2, C11O6H19N2, respectively. The mass of the second glycan component was determined by subtracting the nonulosonate fragment ion from the intact glycan ion (m/z 451.1922 – m/z 275.1231 = 176.0691 Da), and the best fitting elemental composition was C7O5H12.