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. Author manuscript; available in PMC: 2018 Jan 1.
Published in final edited form as: Mol Microbiol. 2016 Oct 27;103(1):67–85. doi: 10.1111/mmi.13544

Fig. 6. Characterizations of the TDE0960 mutant (Tde960mut) and its complemented strain (Tde960com).

Fig. 6

(A) Immunoblotting analysis of the wild-type, Tde960mut, and Tde960com strains. Equivalent amounts of whole cell lysates were analyzed by SDS-PAGE and then probed with a specific antibody against TDE0960. DnaK was used as a loading control. (B) Glycosylation staining analysis of the whole cell lysates. The analysis was carried out as described above. (C) The levels of FlaA and FlaBs were significantly decreased in the Tde960mut strain. Equivalent amounts of the whole cell lysates were analyzed by SDS-PAGE and then probed with the antibodies of DnaK, FlaA, FlaBs, or FlgE. (D) Different amounts of whole cell lysates (as indicated on the figure) were analyzed by SDS-PAGE and then probed with the FlaA and FlaB antibodies.