Table 1.
Glycopeptides identified by nLC-MS/MS analysis of T. denticola flagellin tryptic digests.
| Protein | Amino acid seq. | Amino acid # | # of 450.2 Da modifications |
|---|---|---|---|
| FlaB1 | MQIQVEVSQLVAEVDR | 108-123 | 1 |
| IASSAQFNGMNMLTGR | 124-139 | 2 | |
| ETGENVVTGSMWFHIGANMDQR | 143-164 | 1 | |
| VYIGTMSAAALGIR | 167-180 | 1 | |
| FlaB2 | LYIQVEVSQLIAEVDR | 108-123 | 1 |
| IASHAQFNGMNMLTGR | 124-139 | 1,2 | |
| FAQETGENTVTASMWFHIGANMDQR | 140-164 | 1 | |
| AYIGTMTAK | 167-175 | 1 | |
| FlaB3* | MQIQVEVSQLVDEVDR | 108-123 | 1 |
| IASHAQFNGMNILTGR | 124-139 | 1,2 | |
| IYIGTMTATALGIIGAQQGGEDK | 163-185 | 1 |
Underlined sequences from FlaB1 and FlaB3 were isolated for NMR analysis.
*
The FlaB3 tryptic peptide, T140-160 (FAQDSVTGPMQLHVGANMDQR), was detected only as a non-glycosylated peptide; whereas the equivalent tryptic peptides from FlaB1 and FlaB2 were detected in glycosylated form only.