Table 1.

Glycopeptides identified by nLC-MS/MS analysis of T. denticola flagellin tryptic digests.

Protein	Amino acid seq.	Amino acid #	# of 450.2 Da modifications
FlaB1	MQIQVEVSQLVAEVDR	108-123	1
	IASSAQFNGMNMLTGR	124-139	2
	ETGENVVTGSMWFHIGANMDQR	143-164	1
	VYIGTMSAAALGIR	167-180	1
FlaB2	LYIQVEVSQLIAEVDR	108-123	1
	IASHAQFNGMNMLTGR	124-139	1,2
	FAQETGENTVTASMWFHIGANMDQR	140-164	1
	AYIGTMTAK	167-175	1
FlaB3*	MQIQVEVSQLVDEVDR	108-123	1
	IASHAQFNGMNILTGR	124-139	1,2
	IYIGTMTATALGIIGAQQGGEDK	163-185	1

Underlined sequences from FlaB1 and FlaB3 were isolated for NMR analysis.

^*The FlaB3 tryptic peptide, T^140-160 (FAQDSVTGPMQLHVGANMDQR), was detected only as a non-glycosylated peptide; whereas the equivalent tryptic peptides from FlaB1 and FlaB2 were detected in glycosylated form only.