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. Author manuscript; available in PMC: 2017 Dec 22.
Published in final edited form as: Cell Chem Biol. 2016 Nov 17;23(12):1504–1514. doi: 10.1016/j.chembiol.2016.10.009

Figure 5.

Figure 5

Gene deletion of amm6 and genetic complementation of pCAP01/ammΔamm6 with various pKY01 constructs. pKY01 is an integrative plasmid for the expression of gene products under the control of a the ermE promotor. Peaks for ammosamides A (1) and B (2) produced by S. coelicolor-M512 pCAP01/amm are indicated. The absence of ammosamide A and B was verified by retention time with respect to the positive control (pCAP01/amm), UV absorbance, and mass spectrometry. Related metabolites could not be identified by UV absorbance or extracting masses.