Table 1.
Predicted function of open reading frames (ORFs) in the amm locus of Streptomyces sp. CNR-698. ORFs capture by transformation associated recombination (TAR) are named amm1–27 and highlighted in grey. For homologues of amm ORFs that are associated with other reported biosynthetic gene clusters, the corresponding natural product is indicated in the “Notes” column.
| Name | Predicted Function | Size (a.a.) | Predicted Function of Closest Homologue | % ID | Notes |
|---|---|---|---|---|---|
| amm1 | (2Fe-2S)-Binding protein | 157 | HP, Streptomyces sp. CNT302 | 98% | |
| amm2 | HP | 486 | HP, Amycolatopsis japonica | 55% | |
| amm3 | Tryptophan halogenase (FAD) | 695 | Microbispora carollina, tryptophan-5-halogenase | 60% | Microbisporicin |
| amm4 | F420 Oxidase | 146 | Amycolatopsis thermoflava, F420 Oxidase | 42% | |
| amm5 | HP | 199 | HP, Streptomyces aurantiacus | 68% | |
| amm6 | HP | 56 | HP, Salinispora, multispecies | 68% | Lymphostin |
| amm7 | HP | 614 | HP, Salinispora, multispecies | 55% | Lymphostin |
| amm8 | LD | 806 | LD, Salinispora, multispecies | 54% | Lymphostin |
| amm9 | LD | 913 | LD, Salinispora, multispecies | 61% | Lymphostin |
| amm10 | HP | 524 | HP, Salinispora, multispecies | 42% | Lymphostin |
| amm11 | LD | 832 | LD, Salinispora, multispecies | 51% | Lymphostin |
| amm12 | P | 419 | P, Salinispora, multispecies | 46% | Lymphostin |
| amm13 | HP | 393 | HP, Salinispora, multispecies | 39% | Lymphostin |
| amm14 | FAD-dependent oxidase | 363 | Salinispora, multispecies, FAD-dep oxidase | 55% | Lymphostin |
| amm15 | HP | 225 | HP, Salinispora, multispecies | 73% | Lymphostin |
| amm16 | Flavoprotein | 199 | Salinispora, multispecies, flavoprotein | 54% | |
| amm17 | Flavoprotein | 212 | Salinispora, multispecies, flavin reductase | 54% | Lymphostin |
| amm18 | LD | 936 | LD, Salinispora multispecies, | 48% | Lymphostin |
| amm19 | P (M20 dipeptidase) | 395 | P (M20 dipeptidase), Salinispora, multispecies | 62% | Lymphostin |
| amm20 | Asparagine synthase | 612 | Salinispora, multispecies, asparagine synthase | 61% | Lymphostin |
| amm21 | T | 526 | T, Kibdelosporangium sp. MJ126-NF4 | 57% | |
| amm22 | HP | 525 | HP, Nocardia vinacea | 43% | |
| amm23 | MT | 356 | MT, Nocardiopsis sp. NRRL B-16309 | 45% | |
| amm24 | TetR regulator | 201 | TetR, Nocardia brevicatena | 56% | |
| amm25 | HP | 254 | Saccharothrix sp. NRRL B-16348, glyoxalase | 50% | |
| amm26 | HP | 284 | HP, Streptomyces sp. CNT302 | 99% | |
| amm27 | HP | 131 | HP, Streptomyces sp. CNT302 | 99% |
(Abbreviations: NC = not captured, HP = hypothetical protein, LD = lantibiotic dehydratase, P = peptidase, MT = Methyltransferase, T = Transporter)