Table 1.
Kinetic parameters of sterol substrates assayed with CrSMT.
| Substratea | Km (μM) |
Vmax (pmol/min/mg) |
Vmax/Km |
|---|---|---|---|
| Cycloartenol | 25 | 137 | 5.48 (100) |
| Lanosterol | 24 | 109 | 4.54 (80) |
| Zymosterol | 22 | 120 | 5.45 (99) |
| 24(28)-Methylene lophenol | 20 | 52 | 2.89 (53) |
| Obtusifoliol | 19 | 42 | 2.21 (40) |
| 24(28)-Methylene cycloartanol | 18 | 29 | 1.61 (29) |
| Ergosta-5,7,25(27)-trienol | 0 | 0 | 0 |
| Cyclolaudenol | 0 | 0 | 0 |
a
Catalytic competence of cycloartenol conversion to product is normalized to 100% (in parenthesis). See Fig. 5 for a key to structures.