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. Author manuscript; available in PMC: 2017 Oct 1.
Published in final edited form as: Eur J Immunol. 2016 Aug 29;46(10):2401–2408. doi: 10.1002/eji.201646440

Figure 5.

Figure 5

Effect of proximal or distal lck promoter expression on peripheral T cells. A, B) Spleen cells from WT, lck-/-, Lck-PROX and Lck-DIST mice were stained with anti-CD4 and anti-CD8 antibodies, and analyzed by flow cytometry. Histograms represent mean +/- SE number of spleen CD4 and CD8 cells. Statistical analysis is presented in Supporting Figure 4. C) Spleen CD 4 cells were sorted from Lck-PROX and Lck-DIST mouse spleens by flow cytometry, protein lysates prepared and used for Western blot analysis. Results are representative of 3 independent experiments. D) T cell proliferation assays were carried with spleen CD 4 cells which were isolated by MAC cell isolation kit from WT, lck-/-, Lck-PROX and Lck-DIST mouse spleens. Results are representative of 3 independent experiments.