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. Author manuscript; available in PMC: 2016 Dec 24.
Published in final edited form as: Methods Enzymol. 2016 Feb 9;574:149–165. doi: 10.1016/bs.mie.2016.01.005

Figure 4. Generation of designer mononucleosomes.

Figure 4

A. Wild-type and symmetrically sumoylated octamers visualized by 15% SDS-PAGE gel run at 200 V for 45 min and stained with Coomassie. B. Wild-type and symmetrically sumoylated octamers visualized by 5% TBE gel run at 180 V for 50 min and stained with ethidium bromide. su= SUMO-3 C47S.