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Schematic approach for serum BK and DABK analyses. Serum of patients with active TB were collected (a) and processed by on-chip nanotrap fractionation to enrich low-molecular-weight peptides (b). BK and DABK were detected by mass spectrometry (MS) (c). BK and DABK molar amounts were measured by normalization of their target peptide MS intensity ratio to that of a stable isotope-labeled internal standard (IS) peptide (d).
