Skip to main content
. Author manuscript; available in PMC: 2018 Mar 1.
Published in final edited form as: Muscle Nerve. 2016 Nov 28;55(3):384–392. doi: 10.1002/mus.25227

Fig. 1. Successful conditional ablation of satellite cells.

Fig. 1

(A) Timeline of experimental protocol indicating treatment either by oral gavage of tamoxifen or vehicle, followed by washout period, number of days casted, and day when the muscle was harvested. (B) Fluorescence-activated cell sorting (FACS) analysis from gastrocnemius and quadriceps muscles in treated mice confirmed satellite cell reduction, i.e., α7-integrin+/NCAM+/CD31−/CD45− cells in tamoxifen-treated Pax7-DTA muscles relative to vehicle-treated Pax7-DTA muscles (compare blue boxes, which display representative cell populations). Image is representative of 4 independent tests. (C) Quantification of FACS analysis shows an average 65% (range 58–78) reduction of satellite cells in tamoxifen-treated muscles compared with vehicle-treated muscles (n=4 per group, P<0.01, plotted as mean + SEM).