Figure 5.

JAK2 is required for Akt activation and SREBP-1c-mediated NLRP3 and IL-1β expression. HGFs were kept as CL or infected by HGPg (20 MOI). Before being kept as CL or infected by HGPg, HGFs were transfected with si-JAK1, 2, or 3. (A) The phosphorylation of Akt and p70S6K in HGF cell lysate after HGPg infection was determined using Western blotting. (B) The expression of NLRP3 and SREBP-1c in HGF cell lysate after HGPg infection was determined using Western blotting. (C) IL-1β secretion in medium was determined by ELISA analyses. The results are shown as mean ± SEM. ^*P < 0.05 vs. CL. ^#P < 0.05 vs. si-CL-transfected HGFs with HGPg infection. (D) The phosphorylation of JAK2 in HGF cell lysate after HGPg infection was determined using Western blotting.