Figure 6.

β1 integrin is required for HGPg-induced NLRP3 expression. HGFs were kept as CL or infected by HGPg (20 MOI). Before being kept as CL or infected by HGPg, HGFs were transfected with si-integrin β1 or β2, or pretreated with neutralizing antibody against integrin β1. (A) The phosphorylation of JAK2 in HGF cell lysate after HGPg infection was determined using Western blotting. (B) The phosphorylation of Akt and p70S6K, and the expression of NLRP3 and SREBP-1c in HGF cell lysate after HGPg infection, were determined using Western blotting. (C) IL-1β secretion in medium was determined by ELISA analyses. The results are shown as mean ± SEM. ^*P < 0.05 vs. CL. ^#P < 0.05 vs. si-CL-transfected or IgG-treated HGFs with HGPg infection.