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. 2016 Dec 26;7:2090. doi: 10.3389/fmicb.2016.02090

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Copyright © 2016 Mendes, Santiago, Toledo, Horta, de Souza, Tasic and de Souza.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) SDS-PAGE of the total secreted proteins from X. fastidiosa strains 9a5c and J1a12. M: molecular weight marker (kDa); 3–30: days of growth; and Ca: 2.5 mmol dm^-3 calcium (II) chloride. (B) Transmission electron microscopy of the outer membrane vesicles (OMVs) of X. fastidiosa strains 9a5c (red arrows indicate same of the OMVs in the photo). (C) Western blot analyses of the total protein inside the OMVs of X. fastidiosa strains 9a5c and J1a12. Proteins were identified using an antibody against Antitoxin (XF2491).