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. 2016 Dec 26;7:1963. doi: 10.3389/fpls.2016.01963

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Copyright © 2016 He, Li, Cui, Xie, Zheng, Zhou, Zhou and Xie.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Analysis of the interaction between OsPIF16 and OsPHYB using a yeast two-hybrid assay. (A) Protein constructs used in the yeast two-hybrid assay. OsPIF16 was used as a prey in a translational fusion with the GAL4 AD. The C-terminal non-photoactive region of rice PHYB was fused with the GAL4 BD to use as a bait. (B) Analysis of protein–protein interactions in yeast growing in medium with (left) or without (right) histidine. The negative control was the interaction between OsPIF16 and GBD.