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. 2016 Dec 26;9:145. doi: 10.3389/fnmol.2016.00145

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Copyright © 2016 Francius, Hidalgo-Figueroa, Debrulle, Pelosi, Rucchin, Ronellenfitch, Panayiotou, Makrides, Misra, Harris, Hassani, Schakman, Parras, Xiang, Malas, Chow and Clotman.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution and reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Onecut (OC)-1 and Vsx1 are present in an early V2 interneuron population. (A–A″′) At e9.5, OC-1 is detected in cells located between motor neurons (Isl1+, inset A′; arrowhead indicates the location of A″ and A″′ insets) and V1 interneurons (Foxd3+). (B–B″′) These cells derive from the p2 progenitor domain (Ascl1+) and are not motor neurons (Isl1+, inset B′; arrowhead indicates the location of B″ and B″′ insets). (C–C″) OC-1 is detected in the V2 domain in cells that are neither V2a (Chx10+) nor V2b (Gata3+) interneurons. (D–D″′) Vsx1 is detected in cells containing OC-1 located between motor neurons (Isl1+, inset D′; arrowhead indicates the location of D″ and D″′ insets) and V1 interneurons (Foxd3+). (E–E″) Vsx1 is present in cells that derive from the p2 domain (Ascl1+) and contain OC-1. (F–F″) Vsx1⁺ cells are distinct from early V2a (Chx10+) and V2b (Gata3+) interneurons. Scale bar = 50 μm.