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Pax6 is necessary for Vsx1 expression. (A–C) The generation of Vsx1+, Chx10 + and Gata3 + cells, is not affected in Oc1−/−Oc2−/− embryos. (D–F) In Pax6Sey/Sey embryos, the number of Vsx1+ cells is dramatically reduced (***p < 0.001) without similar alteration of V2a or V2b production. (G–I) In embryos lacking Vsx1, the production of V2a and of V2b interneurons is unaffected. Scale bars = 50 μm. Quantifications are expressed as mean values ± SEM, n = 3.
