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. 2016 Dec 26;7:2117. doi: 10.3389/fmicb.2016.02117

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Copyright © 2016 Gundogdu, da Silva, Mohammad, Elmi, Wren, van Vliet and Dorrell.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Galleria mellonella infection model on C. jejuni 11168H, 81-176, 81116, and M1 wild-type strains and respective rrpA and rrpB mutants. G. mellonella larvae were injected with a 10 μl inoculum of a 24 h C. jejuni culture diluted to OD₆₀₀ 0.1 by micro-injection in the right foremost leg, giving an infectious dose of approximately 10⁶ CFU. Larvae were incubated at 37°C with survival and appearance recorded at 24 h intervals. PBS and no injection controls were used. For each experiment, 10 G. mellonella larvae were infected and experiments were repeated in triplicate. Asterisks denote a statistically significant difference (^∗ = p < 0.05) between wild-type and mutant strains.