Figure 4.

mRNA gene expression changes in NuLi-1 cells at 24 h of WCH-SK2^WT and WCH-SK2^SCV intracellular infection. The cells were incubated with S. aureus for 6 h (MOI 100), followed by lysostaphin treatment. The cells were incubated with gentamycin for another 17.5 h prior cell lysis and RNA extraction. The values represent the mean ± SD of four independent experiments measured. ^*p ≤ 0.05, n.s. = non-significant, two tailed t-test with permutation between either WCH-SK2^WT or WCH-SK2^SCV vs. negative controls. WCH-SK2^WT = Staphylococcus aureus strain WCH-SK2 wild type. WCH-SK2^SCV = Staphylococcus aureus strain WCH-SK2 small colony variant.